Home / Application Notes / CRISPR/Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking

CRISPR/Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking

316 CRISPR/Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking
Carl White1,2, Ethan See1,2, Kevin Pfleger1,2,3 1Molecular Endocrinology and Pharmacology, Harry Perkins Institute of Medical Research, Australia, 2Centre for Medical Research, The University of Western Australia, Australia, 3Dimerix Limited, Nedlands, Australia

In this note the utility of nanoBRET to monitor protein interactions is enhanced when gene editing is performed with CRISPR/Cas9. Specifically endogenous GPCR; CXCR4 was tagged with Nluc in HEK293FT cells. To monitor receptor-trafficking, these cells were then transfected with fluorescent-tagged K-ras and Rab4.

The flexibility and sensitivity of the CLARIOstars LVF monochromator is key to performing some of these assays.

Applicable Products

The following BMG Labtech microplate reader products are applicable.