In this note the utility of nanoBRET to monitor protein interactions is enhanced when gene editing is performed with CRISPR/Cas9. Specifically endogenous GPCR; CXCR4 was tagged with Nluc in HEK293FT cells. To monitor receptor-trafficking, these cells were then transfected with fluorescent-tagged K-ras and Rab4.
The flexibility and sensitivity of the CLARIOstars LVF monochromator is key to performing some of these assays.
The following BMG Labtech microplate reader products are applicable.