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FRET sensor assesses ATP levels in living plants experiencing low oxygen conditions

318 FRET sensor assesses ATP levels in living plants experiencing low oxygen conditions
S Wagner, P Fuchs, T Nietzel, M Elsasser, M Schwarzlander; Institute of Plant Biology and Biotechnology, University of Munster, Germany and Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Germany

A plant based system was used to show the performance of a sensor that detects MgATP. The ATeam sensor is built from the binding domain of the epsilon subunit of ATP synthase flanked by CFP and Venus fluorescent protein. Binding of MgATP brings the fluorescent protein into close proximity and enables FRET. The LVF monochromator on the CLARIOstar has the sensitivity needed to detect FRET signals such as this.

Applicable Products

The following BMG Labtech microplate reader products are applicable.