Home / Application Notes / NanoBRET assay quantitatively evaluates VEGF binding to the VEGFR2 in real time in living cells

NanoBRET assay quantitatively evaluates VEGF binding to the VEGFR2 in real time in living cells

317 NanoBRET assay quantitatively evaluates VEGF binding to the VEGFR2 in real time in living cells
Kilpatrick LE 1, Friedman-Ohana R 2, Alcobia DC 1, Riching K 2, Peach CJ 1, Wheal AJ 1, Briddon SJ 1, Robers MB 2, Zimmerman K 2, Machleidt T 2, Wood KV 2, Woolard J 1, Hill SJ 1; 1) Division of Physiology, Pharmacology and Neuroscience, University of Nottingham, UK; 2) Promega Corporation, Madison, WI, USA

A novel labeling technique was used to create a system where VEGFR2 binding kinetics could be studied in real time using NanoBRET. The PHERAstar FSX assists in performing the rapid readings required by using its SDE capability to capture both donor and acceptor signals simultaneously.

Applicable Products

The following BMG Labtech microplate reader products are applicable.