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Real-Time Assessment of Apoptosis and Necrosis

311 Real-Time Assessment of Apoptosis and Necrosis
Kevin Kupcho(1), Carl Peters (2) and Andrew Niles (1); 1) Promega Corporation, Madison, WI; 2) BMG LABTECH Inc, NC, USA

Although cell death is an important, normal consequence of multicellular homeostasis it also has an unfortunate role in many human diseases. Discovery of targeted cytotoxic and cytoprotective agents may therefore have important implications in treating cancer, neurodegeneration and a litany of other diseases. In order to fully appreciate the dysregulation in a disease model, and responses to experimental stimuli, it is important establish the mechanism of action of cell death and define response kinetics. Traditional experimental approaches for addressing mode of cell death are often time consuming, labor intensive, costly and inadequate.

Here we describe the monitoring of apoptosis and necrosis in real time. Promega’s new RealTime-GloTM Annexin V Apoptosis and Necrosis Assay provides the simplicity of an add-mix-measure assay, in a livecell, real-time format…

Applicable Products

The following BMG Labtech microplate reader products are applicable.