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NEPHELOstar Plus

The first and only laser-based nephelometer

BMG LABTECH’s NEPHELOstar Plus is truly in a class by itself. It employs a laser to detect forward scattering of light to insoluble particles in a sample. This process, laser based nephelometry, is not unique but incorporating this technology into a plate reader is!

So whether you are interested in testing compound solubility, performing turbidity measurements or detecting microbial growth with high sensitivity the NEPHELOstar Plus allows you to do all of these in a high-throughput fashion.

Technologies

Features

  • Laser with adjustable intensity and beam width
  • Capable of reading 384-well plates
  • Injectors featuring reading at time of injection
  • ACU compatible
  • 21 CFR compliant software
  • MARS data analysis
  • Stacker compatible

Nephelometry is a means of detecting insoluble particles or cloudiness in a particular sample by passing a light beam through the sample. As the light beam comes into contact with a particle light scattering occurs. More insoluble particles in the sample equates to more extensive light scattering. To quantify the amount of light scattering a system which detects forward light scattering is employed. Nephelometry can be up to 30 times more sensitive than traditional optical density readers!

The NEPHELOstar plus uses a high intensity laser diode whose diameter and intensity can be adjusted so that it can be used for detection of light scattering in plates with up to 384 well! This is commonly used to perform drug solubility screening in high throughput but has also been applied to protein-protein interactions such as a protein-antibody interaction that could be observed in an immuneprecipitation.

Every NEPHELOstar Plus can regulate temperature and for microbial growth experiments it can even be equipped with an Atmospheric Control Unit! This will allow you to maintain the optimal growth environment for your microbe or study the effects of a hypoxic environment.

Detection Modes 

Nephelometry; Light Scattering

 
Measurement Modes 

Endpoint and kinetic measurements

 
Microplate Formats 

Up to 384-well plates

 
Microplate Carrier 

Robot compatible

 
Light Sources 

Self-monitoring laser diode
Wavelength 635±10 nm
Stability <0.2% deviation
Lifetime 20,000 hours
Output: 1 mW
Selectable beam width: 1.5 to 3.5 mm
Selectable Intensity 0-100%
Scattering angle: Detects up to 80° full cone angle

 
Detectors 

Side window photodiode detector

 
Wavelength Selection 

Photodiode
Wavelength 635±10 nm

 
Sensitivity* 

Depends on particle size and liquid properties
Silica detection (particle size 0.5 to 10 µm) 800 nM

Dynamic Range: 5 decades
Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second)

 
Read Times 

Depend on assay conditions and liquid surface stability
16 s (96), 47 s (384) (shortest possible times)

 
Reagent Injection 

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

 
Shaking 

Linear, orbital, and double-orbital with user-definable time and speed

 
Purge Gas Vent 

System to inject an atmosphere or to pull a vacuum into the reader

 
Incubation 

+4°C above ambient up to 45 °C

 
Software 

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

 
Dimensions 

Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg

 
Atmospheric Control Unit (ACU) (Optional Accessory) 

Oxygen Regulation: 1 – 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 – 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg

 
Stacker (Optional Accessory) 

Magazines for up to 50 plates – continuous loading feature

 
THERMOstar (Optional Accessory) 

Microplate Incubator and Shaker

 
Upgrades (Optional Accessory) 

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.