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Microplate readers are complex scientific research instruments that measure thousands of different applications.

BMG LABTECH has become a world leader in microplate reading systems due to continuous integration of new and unique technology.


Absorbance

Absorbance-based detection can be performed on the PHERAstar® FSX, CLARIOstar®, FLUOstar®, SPECTROstar® and POLARstar® Omega plate readers, as well as on the dedicated SPECTROstar Nano. A high-power xenon flash lamp illuminates the sample using a specific wavelength selected by an optical filter. In the case of most BMG LABTECH microplate readers, a UV/Vis spectrometer is …

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Fluorescence (including FRET)

Fluorescence intensity detection is the measurement of the light emitted by a fluorescent molecule (fluorophore) upon excitation by light at a higher energy and smaller wavelength. A sample is excited by the light produced by a light source and filtered at a specific wavelength by either a filter or a monochromator. The excited sample emits …

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Luminescence (including BRET)

Dedicated high performance luminescence Luminescence is a key detection mode for microplate readers, both in the academic and high throughput screening setting. Analytically luminescence can be the most sensitive assay mode available, but the assays can be challenging and demanding on microplate instrumentation. All BMG LABTECH microplate readers, from our entry level OMEGA platform, up …

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Time-Resolved Fluorescence (HTRF® & TR-FRET)

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a special form of resonance energy transfer (FRET) in which long emission fluorophores (lanthanides) are used as donors. The comparison measurement of the two emitted wavelengths over time is calculated for a TR-FRET response. The most common TR-FRET assays include HTRF®, LanthaScreen®, LANCE®, and Transcreener® ADP2 TR-FRET Red …

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Fluorescence Polarization

Fluorescence polarization is similar to fluorescence intensity, in that it is based on the emission of light by an excited fluorophore. However, samples are excited by polarized light which is selected by specific polarizing filters. Depending on the mobility of the fluorescent molecules in the wells, the emission light will either be polarized or not. …

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AlphaScreen®

Amplified Luminescent Proximity Homogeneous Assay (Alpha) Technology, commonly known as AlphaScreen® and AlphaLISA®, is a bead-based detection mode which measures the interaction of two molecules bioconjucated to donor and acceptor beads. When excited at 680 nm, the donor bead produces singlet oxygen. If the acceptor bead is within a 200 nm distance, the singlet oxygen …

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Nephelometry

Nephelometry is a method for the determination of the cloudiness of a solution. A light beam passes through the sample and the light is scattered by the suspended particles. The measured forward scattered light indicates the amount of the insoluble particles present in solution. Common nephelometry applications include automated HTS drug solubility screening, long-term microbial …

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