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Microplate readers are complex scientific research instruments that measure thousands of different applications.

BMG LABTECH has become a world leader in microplate reading systems due to continuous integration of new and unique technology.


Absorbance

Absorbance-based detection can be performed on the PHERAstar® FSX, CLARIOstar®, FLUOstar®, SPECTROstar® and POLARstar® Omega plate readers, as well as on the dedicated SPECTROstar Nano. A high-power xenon flash lamp illuminates the sample using a specific wavelength selected by an optical filter. In the case of most BMG LABTECH microplate readers, a UV/Vis spectrometer is …

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Fluorescence (including FRET)

Fluorescence intensity is one of the most common measurement techniques in microplate readers. Conceptually it is a simple, a fluorophore is excited at one wavelength and emits light at another wavelength. The emitted light is at a lower energy and thus longer wavelength than the excitation light, and, the amount of emission is directly related …

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Luminescence (including BRET)

Dedicated high performance luminescence Luminescence is a key detection mode for microplate readers, both in the academic and high throughput screening setting. Analytically luminescence can be the most sensitive assay mode available, but the assays can be challenging and demanding on microplate instrumentation. All BMG LABTECH microplate readers, from our entry level OMEGA platform, up …

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Time-Resolved Fluorescence (HTRF® & TR-FRET)

Time-Resolved Fluorescence (TRF) takes advantage of a particular characteristic of the elements called lanthanides. These fluorophores have long emission lifetimes that allow for changes to be made regarding when detection starts and how long emission is measured. Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) incorporates lanthandies as donors in a unique form of resonance energy transfer …

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Fluorescence Polarization

Fluorescence polarization (FP) is a homogenous assay technique that can be used to detect the interactions, or binding, between two molecules. FP relies on the differences in signal intensity between orthogonal polarizations of light to determine the relative rotational speed of molecules – i.e., unbound molecules rotate faster than bound molecules and the FP assay …

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AlphaScreen®

Amplified Luminescent Proximity Homogeneous Assay (Alpha) Technologies are commonly known as AlphaScreen® and AlphaLISA® and more recently AlphaPlex®, This detection method measures interaction between two molecules due to their modification with donor and acceptor beads.  The donor bead produces singlet oxygen when excited at 680 nm. The singlet oxygen transfers energy to the acceptor resulting …

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Nephelometry

Nephelometry is a very sensitive optical measurement technique that can be used to detect suspended particles in solution. The basics of nephelometry involve passing a laser beam through a liquid sample in a microplate reader and measuring the amount of light that is scattered by using an Ulbricht sphere. Generally speaking, as the suspended particle …

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