Amplified Luminescent Proximity Homogeneous Assay (Alpha) Technology, commonly known as AlphaScreen® and AlphaLISA®, is a bead-based detection mode which measures the interaction of two molecules bioconjucated to donor and acceptor beads. When excited at 680 nm, the donor bead produces singlet oxygen. If the acceptor bead is within a 200 nm distance, the singlet oxygen transfers energy to the donor resulting in production of light between 520 and 620 nm. The amount of produced light is directly proportionate to the amount of bound donor-acceptor beads.
AlphaLISA is slightly different from AlphaScreen in that the primary emission occurs at 615nm. While AlphaScreen optics can be used to read AlphaLISA, BMG LABTECH supplies specific AlphaScreen and AlphaLISA filters for any reader we equip with Alpha reading technology to improve your assay performance.
AlphaScreen detection with BMG LABTECH microplate readers
BMG LABTECH has been building AlphaScreen detection into our microplate readers since 2005, long before our competitors. AlphaScreen and AlphaLISA detection is an option on the PHERAstar FSX, CLARIOstar, and OMEGA platform. The the PHERAstar FSX and CLARIOstar have solid-state lasers at 680nm for excitation, and combined with the superior low-noise luminescent performance of these microplate readers they are excellent choices for laboratories performing HTS/1536/3456 or in need high quality data with AlphaScreen®, AlphaLISA®, and SureFire® assays. The OMEGA is a strong academic Alpha technology platform and well-suited for 96 well AlphaScreen/AlphaLISA assays.
Selected Alpha technology application notes
- AN 153: Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode
- AN 260: Miniaturization of a Cell-based TNF-α AlphaLISA® Assay Using Echo® Liquid Handler and the PHERAstar FS
- AN 210: An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on BMG LABTECH’s POLARstar Omega
See a complete list of Alpha Technology application notes.
The following BMG LABTECH microplate readers can be configured to perform Alpha Technology measurements.