Fluorescence polarization (FP) is a homogenous assay technique that can be used to detect the interactions, or binding, between two molecules. FP relies on the differences in signal intensity between orthogonal polarizations of light to determine the relative rotational speed of molecules – i.e., unbound molecules rotate faster than bound molecules and the FP assay can be used to determine differences between bound and unbound states. FP can be used with dozens of fluorescent tags from common fluorescein/FITC to UV fluorophores and even out to the far red with Cy dyes and other variants.
Historically fluorescence polarization has been a challenging application to run for most laboratories. But the PHERAstar, and now CLARIOstar, arguably two of the most sensitive FP readers available, have changed the role of FP in the modern life science lab. FP has seen a resurgence due to their superior optical designs as these two microplate readers take the difficulty out of FP experiments, instrument setup and optimization, allowing scientists to focus on experimental design.
Fluorescence polarization detection with BMG LABTECH microplate readers
Fluorescence polarization can be performed on the PHERAstar® FSX, CLARIOstar®, and OMEGA series microplate readers. A popular application for FP is the Predictor hERG assay, however, the readers are capable of running any functional FP assay that is used in a laboratory. Have a look though our keyword searchable applications database for FP assays of interest or contact one of our applications scientists for more information.
Examples of fluorescence polarization measurements on BMG LABTECH instruments
- AN 180: High-throughput protein-DNA affinity measurements using fluorescence anisotropy
- AN 205: Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
- AN 244: The CLARIOstar® surpasses the certification needed for the Transcreener® ADP² FP assay
See a complete list of fluorescence polarization application notes.
The following BMG LABTECH microplate readers can be configured to perform fluorescence polarization measurements.