Nephelometry is a very sensitive optical measurement technique that can be used to detect suspended particles in solution. The basics of nephelometry involve passing a laser beam through a liquid sample in a microplate reader and measuring the amount of light that is scattered by using an Ulbricht sphere. Generally speaking, as the suspended particle density increases the amount of light scattered will increase thus driving the nephelometry signal to higher values. The laser beam intensity and focus can be adjusted as needed depending on the experimental environment or application. Nephelometry is extremely sensitive and can outperform more common turbidity measurements or optical density determinations based on the OD600 techniques.
Nephelometry detection with BMG LABTECH microplate readers
BMG LABTECH produces the world’s only laser-based microplate reader nephelometer, the NEPHELOstar® Plus. The NEPHELOstar Plus can be used for a wide variety of light scattering measurements such as long-term microbial growth studies, high throughput screening (HTS) drug solubility applications, immunoprecipitation, and studies involving polymerization and precipitation of target molecules.
The NEPHELOstar Plus can now be equipped with BMG LABTECH’s OMEGA series Atmospheric Control Unit (ACU) for O2 and CO2 regulation thus greatly expanding the application possibilities. We have common NEPHELOstar Plus applications listed below but if you have questions about your specific application call and speak to one of our scientists who can certainly assist you.
Examples of nephelometry measurements on BMG LABTECH instruments
- AN 238: 96-well nephelometric assay to detect calcification propensity of serum samples
- AN 174: Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH’s NEPHELOstar
- AN 133: Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader
See a complete list of nephelometry application notes.
The following BMG Labtech microplate reader can be configured to perform nephelometry measurements.