Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a special form of resonance energy transfer (FRET) in which long emission fluorophores (lanthanides) are used as donors. The comparison measurement of the two emitted wavelengths over time is calculated for a TR-FRET response. The most common TR-FRET assays include HTRF®, LanthaScreen®, LANCE®, and Transcreener® ADP2 TR-FRET Red Assay.
Time-resolved fluorescence detection with BMG LABTECH microplate readers
TR-FRET can be performed on the PHERAstar® FSX, CLARIOstar®, and SPECTROstar® Omega which are certified for HTRF®, LanthaScreen®, and Transcreener® ADP2 TR-FRET Red Assay. A high-intensity xenon flashlamp provides high performance measurements, while assay-specific filters or optic modules select the specific wavelengths, yield higher light transmission, and minimize unwanted background noise. The PHERAstar FS offers further TRF-dedicated features such as the unique Decay Curve Monitoring and a dedicated TRF-laser.
Examples of TR-FRET measurements on BMG LABTECH instruments
- AN 236: Identification and characterisation of novel positive allosteric modulators of the Galanin 2 Receptor
- AN 222: HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway
- AN 209: GPCR Activation is Measured Using Cisbio’s cAMP and IP1 HTRF® HTplex™ Cell-based Assay
See a complete list of TRF and TR-FRET application notes.
The following BMG Labtech microplate readers can be configured to perform time-resolved fluorescence measurements.